The Global Standard for Breath Biomarker Collection
RTube™ is the most trusted, validated and widely adopted Exhaled Breath Condensate collection platform — delivering standardized, scalable, reproducible sampling for clinical trials and occupational exposure monitoring.
230,000+
Collections Worldwide
400+
Peer-Reviewed Studies
25+
Years Trusted
ISO 13485
Cleanroom Manufacturing
Why Breath Biomarkers Matter
Exhaled Breath Condensate (EBC) provides a non‑invasive window into airway biology. RTube™ captures both dissolved and aerosolized components of exhalate, enabling analysis of a wide range of biomarkers including:
- DNA, RNA, microRNA
- Proteins & cytokines
- Oxides of nitrogen
- pH, hydrogen peroxide, and VOCs
- Heavy metals
- 8-isoprostane and other arachidonic acid metabolites
RTube™ offers the most practical, accessible, scalable entry point into breath‑based biomarker research.
Engineered for Reproducible, Contamination‑Free Sampling
Your biomarker data depends on clean, consistent samples. RTube™ is built to minimize pre‑analytical variability and protect biomarker integrity from collection through analysis.
- ISO 8 cleanroom manufacturing
- ISO 13485 certified facility
- Controlled cleaning and manufacturing to prevent chemical and organic contamination
- Sealed, single‑use disposable design
- Collect, store, transport, and analyze within one device to avoid transfers
- Validated collection protocols
The result: cleaner samples, lower analytical risk, and more reproducible data across sites, operators, and environments.
Scalable for Real‑World Clinical Research
RTube™ supports modern respiratory research across diverse environments from academic medical research, decentralized pharmaceutical clinical trials and population health studies to occupational exposure monitoring and industrial hygiene programs. Low operational burden enables efficient decentralized and field-based studies at scale.
- Easy for unsupervised patient use
- No cold chain required
- Minimal training load
- No cleaning or supporting equipment
- Simplified procurement & logistics
RTube™ Platform Engineered for Distinct Workflows
RTube
Exhaled Breath Condensate Collector
Disposable hand-held breath condensate collector for use any time, any where.
RTubeVOC
End Tidal Air Collector for Volatile Organic Compounds.
Disposable single-breath collector selectively captures the last 65 ml of alveolar exhalate.
RTubeVENT
Exhaled Breath Condensate Collector for Intubated Patient Use.
Disposable breath condensate collector for in-line use in ventilator expiratory limb.
Publications
Advancements in EBC research using RTube
Explore the forefront of respiratory research with the RTube exhaled breath condensate collector.
Starter Kits – Get Preliminary Data Fast
All‑inclusive kits designed to accelerate early‑stage research and feasibility studies.
RTube™
25 Collection Starter Kit- Breath Condensate
- Ambulatory Population
RTubeVOC™
25 Collection Starter Kit- Gas-Phase Breath
- Ambulatory Population
RTubeVENT™
25 Collection Starter Kit- Breath Condensate
- Intubated/Ventilated Population
Full Support from Protocol to Publication
From protocol development to publication, our team provides hands-on support for study methods, logistics planning, and site training.
How We Manufacture the RTube™
Because even trace contaminants can skew biomarker data, Respiratory Research, Inc. employs a proprietary cleaning process that removes both chemical residues and organic matter. Manufactured in an ISO 8 cleanroom, each RTube™ is sealed to guarantee pristine breath analysis—ensuring accurate breath biomarkers with every use.
Effectiveness of Exhaled Breath Condensate Collection Using RTube® and Cooling Sleeve Chilled in Household Freezer
Chilled in a household freezer, the RTube® reliably collects Exhaled Breath Condensate for breath analysis and breath biomarkers. This study shows yields remain near-constant across a wide temperature range, reinforcing RTube’s consistent performance in real-world clinical conditions.
Journal Article Summary – Measuring RNA in Breath Condensate
The RTube has successfully been used to isolate RNA, microRNA, and DNA from Exhaled Breath Condensate (EBC). While the collection process of EBC is similar for each target, the isolation steps differ. This Journal Article Summary, based on Dr. Guillermo Barreto’s EMBO Medicine study, highlights a non-invasive approach to nucleic acid isolation for early-stage lung cancer detection.