Exhaled breath condensate (EBC) is a non-invasive tool that provides airway-level biological context in early-phase respiratory drug development, helping to distinguish whether ambiguous trial results are due to drug failure or inadequate measurement. EBC is most valuable when the pharmacodynamic question is lung-compartment specific and cannot be answered by blood or FeNO, but its biomarkers are exploratory and require a pre-specified analysis plan for meaningful interpretation. While EBC does not provide validated endpoints, its inclusion can inform critical development decisions by adding mechanistic insight that systemic measures alone cannot offer.
How To
Stay updated on groundbreaking announcements, product launches, and important developments in respiratory research. Our press releases cover everything from new breath biomarker discoveries to RTube™ device updates, providing timely insights into the evolving world of breath analysis.
How We Manufacture the RTube™
Because even trace contaminants can skew biomarker data, Respiratory Research, Inc. employs a proprietary cleaning process that removes both chemical residues and organic matter. Manufactured in an ISO 8 cleanroom, each RTube™ is sealed to guarantee pristine breath analysis—ensuring accurate breath biomarkers with every use.
Effectiveness of Exhaled Breath Condensate Collection Using RTube® and Cooling Sleeve Chilled in Household Freezer
Chilled in a household freezer, the RTube® reliably collects Exhaled Breath Condensate for breath analysis and breath biomarkers. This study shows yields remain near-constant across a wide temperature range, reinforcing RTube’s consistent performance in real-world clinical conditions.
Journal Article Summary – Measuring RNA in Breath Condensate
The RTube has successfully been used to isolate RNA, microRNA, and DNA from Exhaled Breath Condensate (EBC). While the collection process of EBC is similar for each target, the isolation steps differ. This Journal Article Summary, based on Dr. Guillermo Barreto’s EMBO Medicine study, highlights a non-invasive approach to nucleic acid isolation for early-stage lung cancer detection.
To Filter or Not To Filter
Examine the pros and cons of filtering exhaled breath samples. Learn how filtration affects sample purity, device performance, and data integrity for biomarker analysis.
An Evolving Standard for Exhaled Biomarkers in Lung Disease Meets Old-Fashioned Consistency
Delve into emerging guidelines that standardize exhaled biomarker collection and analysis. Understand how they shape the future of respiratory diagnostics and research.
Tips and Methods: Collect EBC and Sample VOC’s Simultaneously
Master the art of collecting Exhaled Breath Condensate (EBC) alongside Volatile Organic Compounds (VOCs). Learn techniques that expand breath biomarker research by capturing both biochemical and volatile signatures in a single session.
A Simple Method for Comparing Cytokine Levels in Breath
Discover a streamlined approach to measure cytokine levels in Exhaled Breath Condensate (EBC), advancing breath biomarker research by simplifying the comparison of inflammatory markers across different breath analyses.
MicroRNA in Exhaled Breath May Help Predict Lung Cancer
Explore how microRNA in Exhaled Breath Condensate (EBC) could provide a non-invasive tool for early lung cancer detection, showcasing breath biomarker breakthroughs that may transform clinical diagnostics.
The Study of Exhaled Breath Condensate pH
Investigate how pH measurement in Exhaled Breath Condensate (EBC) can gauge respiratory inflammation and disease progression, supporting early diagnosis and tailored treatment.
Why use the RTube if I already have an EcoScreen?
Already have an EcoScreen? Discover why the RTube® remains the leading choice for Exhaled Breath Condensate collection—ensuring consistent yields, minimal contamination risk, and reliable results for breath biomarker research.
Cytokines in Exhaled Breath Condensate (EBC) – Part 1
Uncover how cytokines in EBC reveal critical biomarkers for respiratory inflammation. This first installment shows how breath analysers and EBC collection can shed new light on disease pathways and targeted therapies.
Tips: Explain Procedure to Your Study Subjects with Video
Ensure consistent EBC data by clearly demonstrating the breath analysis procedure to study subjects. A concise how-to video helps participants follow protocols correctly, boosting reliability for breath biomarker research.
How To: Precautions to Eliminate the Risk of Insulating Sleeve Cross-Contamination
See how the RTube® helps prevent cross-contamination in breath analysis. Learn about its design features that safeguard patients and ensure accurate biomarker results.

